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KMID : 0984720030350010001
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Infection and Chemotherapy
2003 Volume.35 No. 1 p.1 ~ p.11
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Detection of Rhinovirus from Children with Lower Respiratory Tract Infections by Reverse Transcription Polymerase Chain Reaction
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Kwak Young-Ho
Choi Eun-Hwa
Lee Hoan-Jong
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Abstract
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Background: Rhinovirus has long been considered as the most important cause of uncomplicated upper respiratory infection and a trigger of asthma exacerbation, and was recently been widely appreciated for it´s association with lower respiratory tract illness.
Methods: One hundred and sixty-six children with lower respiratory tract infections were enrolled. They had been hospitalized at the Seoul National University Children´s Hospital from August 1997 to March 2000. All the children had been previously healthy and under 5 years of age. Rhinovirus was detected in nasal aspirates by semi-nested reverse transcription polymerase chain reaction and confirmed by digestion pattern of the amplicon with a restriction enzyme. Presence of other respiratory pathogens was also analysed.
Results: Of the 166 children, rhinoviruses were detected in 8 children (4.8%). Majority of rhinovirus was detected in the patients who had been hospitalized in the late fall and winter, and all the infected children recovered completely. Other respiratory pathogens identified were; mycoplasma in 7 (4.2%), respiratory syncytial virus in 15 (9.0%), parainfluenza virus in 8 (4.8%), adenovirus in 4 (2.4%) and influenza virus in 3 (1.8%).
Conclusion: Rhinovirus may be a significant cause of acute lower respiratory infections in healthy children. Further investigation on the diagnosis and clinical characteristics of rhinoviral infection is warranted.
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KEYWORD
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rhinovirus, respiratory infection, polymerase chain reaction
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